UPenn_MRSEC_2309043_IRG2_Good_Chenoweth_Patel_Peptide_Coacervate_Hubs_fig

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A collage of images of coacervates: Top row: loaded with mCherry alone (no nanobody) appear as magenta particles, and the GFP target remains diffuse in the cytosol (no green signal at particles). Bottom row: coacervates loaded with the VHHGFP4–mCherry nanobody concentrate GFP onto the particle surface, producing colocalized magenta + green (white in the merge).

Peptide coacervates loaded with the VHHGFP4 nanobody recruit a cytosolic GFP target inside cells. Top row: coacervates loaded with mCherry alone (no nanobody) appear as magenta particles, and the GFP target remains diffuse in the cytosol (no green signal at particles). Bottom row: coacervates loaded with the VHHGFP4–mCherry nanobody concentrate GFP onto the particle surface, producing colocalized magenta + green (white in the merge). Scale bars 10 µm.