Oleosin variants were made with standard processing techniques from recombinant biotechnology. A. Phase behavior of a series of oleosin mutants, with 65 residues in the hydrophobic core and various lengths of the hydrophilic fraction, that assemble into various structures in solutions of different osmotic strength including B. fibers, C. sheets, and D. vesicles (Vargo et al. 2012), all characterized by cryo-TEM.